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After washing 3 times for 10 mins in PBS, slices were incubated at RT on a shaker for 2 hours in secondary antibodies: goat anti-rabbit Alexa Fluor 594 (1:1000, Invitrogen, A11037) for MBP, goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, A11034) for Iba-1 and donkey anti-mouse Alexa Fluor 594 (1:1000, Thermo Fisher, A21203) for GFAP

Antioxidant and anti-inflammatory activities of berberine in the treatment of diabetes mellitus

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In summary, while women generally possess stronger immune function than men due to hormonal and genetic advantages, this disparity diminishes after menopause as estrogen levels decline