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Our lab previously determined the crystal structures of ME2, revealing that the structure of the ME2_R67Q mutant was an inactivating dead form of the enzyme, whereas the structure of ME2_R484W was an overactivating closed form 44

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1): where is the initial velocity of the reaction, is the maximum velocity achieved by the reaction at maximum concentrations of substrate, is the concentration of substrate, and is the substrate concentration at which the reaction velocity is half of the (Michaelis-Menten constant)

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