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Materials and methods Cell culture Human HCC cell lines HepG2 and PLC/PRF/5 were acquired from the American Type Culture Collection (ATCC, VA, USA)

Colonies were collectively resuspended in 200 mL LB/Amp medium, and 50 mL of this suspension was subjected to plasmid DNA extraction (Midiprep Kit

2.5 Preparation of agar hydrogel containing GSNO Two grams of agar were dissolved in 100 mL of 0.1 M phosphate buffer at pH 7.4 at 80C for 3 h with stirring

Including blocking agents like BSA or non-specific proteins can also help reduce non-specific binding

J Assist Reprod Genet 30:14211429 Ding F, Li Y, Hou X, Zhang R, Hu S, Wang Y (2016) Oxymatrine inhibits microglia activation via HSP60-TLR4 signaling

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