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To ensure adherence to ARRIVE guidelines, additional details relevant to animal research reporting are specified as follows: Randomization: All 60 Sprague-Dawley rats were randomly allocated to 6 experimental groups ( n = 10 per group) using a computer-generated random number table, with allocation concealed by an independent researcher not involved in animal treatment or outcome assessment

Clinical trials were conducted with imatinib, a PDGFR inhibitor, on patients with glioblastoma multiforme (GBM) who were refractory to chemotherapy and radiation therapy

The compound is classified as having extremely low toxicity, with preclinical LD50 values exceeding 5,000 mg/kg in rodent models

Microscopy The tissue specimens were immediately fixed in buffered formalin (pH 7.4), for 24 hours, dehydrated, and embedded in paraffin wax

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Bacteriostatic water limits bacterial growth but does not eliminate it entirely under poor technique