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For Cyss depletion, cells were washed with phosphate-buffered saline solution (PBS), pH 7.4, and incubated for the indicated times in DMEM, high glucose, no glutamine, no methionine, no cystine (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS), 0.2 mM l-Methionine, 100 g/ml streptomycin, 100 U/ml penicillin, and 2 mM l-glutamine

These data demonstrate that GGCT enzymatically diverts Gln flux away from the TCA cycle, preferentially shunting Gln toward GSH synthesis to sustain redox homeostasis

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New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure

Spearman, P