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The cells were lysed by ultrasound for 20 min at 4 C, followed by centrifugation at 12,000 rpm for 15 min to collect the supernatant

Phytochemical identification and analysis GC/MS analysis The n -hexane extracts of the Araucaria heterophylla and Azadirachta indica were injected into a gas chromatography coupled to mass spectrometry (Shimadzu GCMS-QP 2010, Kyoto, Japan) operating in EI mode at 70 eV, and mass spectrum acquisition was performed in the mass range of 35500 amu

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doi: 10.3389/fimmu.2021.734689 108 BertinettoFEMagistroniPMazzolaGACostaCElenaGAlizziSet al