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To assess cell viability, 10 l CellTiter-Fluor (Promega) was added to black plates and incubated for 30 min in the dark before fluorescence was measured at 7-mm depth (gain 100)

On the other hand, there is no doubt that in rat models of cocaine use disorder (CUD), NAC did not change cocaine reward, but effectively reduced escalation of drug intake, cocaine-seeking, and reinstatement behaviors [8, 10, 14, 19, 21, 50,51,52,53,54,55,56,57] (Table 1)

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These data clearly show that choline intake may be beneficial for pregnancies complicated by placental insufficiencies such as preeclampsia or fetal growth restriction