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41 (suppl

EdU incorporation was finally quantified by using a fluorescence microplate reader using an excitation of 568 nm and an emission of 585 nm

Heidelberg: Springer Science+Business Media, LLC

While the needle puncture injury method provides reliable and consistent experimental disc degeneration models, findings from these studies need to be carefully interpreted and considered in a context dependent manner as the animal caudal spine differs in anatomy, mechanical load, and degrees of motion as compared to the human spine