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Some studies showed that secreted soluble Klotho levels decrease before FGF23 levels increase (33,34) but the sequence of events may differ depending on experimental models and diverse clinical conditions (35)

( e ) relative gene expression changes in two independent AKP organoid lines cultured in 3D 5FU for 48 h

Laboratory Study Number:39664 TAR

store in sealed containers with additional desiccant if needed Stability data: 12+ months at -20C, 24+ months at -80C for optimal activity retention Record storage date, conditions, and any temperature excursions in laboratory records pH and Buffer Considerations: Both peptides stable at physiological pH (7.0-7.4) Phosphate buffered saline (PBS, pH 7.4) suitable for most applications HEPES buffer (10-25 mM, pH 7.2-7.4) alternative for CO-independent systems Avoid extreme pH (9.0) which may cause degradation or aggregation For long-term storage, slightly acidic pH (6.0-6.5) may enhance stability for some peptides Stability Considerations: Both peptides carry modifications that improve protease stability (semaglutide via a DPP-4-resistant Aib8 substitution

100 g/mL) for another 24 h