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Dehydration and electrolyte imbalance: Dehydration and depletion of essential minerals such as potassium, magnesium, and calcium can trigger muscle spasms, especially during or after intense physical activity in hot weather

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Membranes were blocked in 5% non-fat dry milk with TBST for 30 min at room temperature and incubated overnight at 4 C with primary antibodies (Supplementary Table 2) diluted in either 5% milk-TBST or 5% bovine serum albumin (BSA)-TBST for phospho-specific antibodies

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