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Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57

Recombinantly-expressed and purified GSTs were used to assess NAPQI binding in vitro

Standard assays may require more than what is available

For specifically IgG2, this difference in transcytosis is not mirrored by a shorter plasma half-life than IgG1, which highlights the complex nuances of FcRn-mediated transport [338]

Yang GM, Tian FY, Shen YW, Yang CY, Yuan H, Li P, Gao ZB (2023) Functional characterization and in vitro pharmacological rescue of KCNQ2 pore mutations associated with epileptic encephalopathy

A species/strain comparison of hepatic natural lymphocytotoxic activities in rats and mice