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Nucleic Acids Res 52:3654 Acknowledgements Not applicable

Although many peptides tolerate routine handling well, gentle mixing is widely regarded as best laboratory practice

Calculation of Dye Amount The amount of dye required for the reaction depends on the amount of protein to be labeled

This is congruent with preclinical data showing that trigeminal and visual input converge at the level of third-order thalamic neurons, which then project to the visual cortex [43, 48]