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SCFAs maintain epithelial barrier integrity, reduce microbial dysbiosis, and exert systemic anti-inflammatory effects

The cells are a major source of fibronectin, but also upregulate many ECM proteins in response to mechanical stressors (pressure, stretch) under both in vivo and in vitro conditions (e.g., tenascin, laminin, collagens, elastin and thrombospondin) (e.g., Figure 14) together with genes associated with cell motility and migration (gelsolin, LIMBP1, periplakin) and downregulation of integrin genes (92, 98)

Furthermore, mRNA and protein expression levels were assessed in the ileum and colon, where a significant increase in the glucotonic effect was observed after metformin treatment

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Each treatment took about 45 minutes from start to finish