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Quantifoil 1.2/1.3 grids were glow-discharged (25 mA, 30 s, 10 1 Pa), 3 l of each sample was deposited on these grids and flash frozen in liquid ethane using an automated plunger (Vitrobot, FEI) with controlled blotting time (1 s), blotting force (1), wait time (0), humidity (100%) and temperature (4 C)

This phenotype is associated with nucleosomal changes at the ribosomal DNA locus, thus affecting rRNA synthesis (Table 1)

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Duan W, Ladenheim B, Cutler RG, Kruman II, Cadet JL, Mattson MP

Annexin V apoptosis assay Apoptosis was measured using the FITC Annexin V apoptosis detection kit II (BD Biosciences, San Diego, CA, USA)