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Moreover, a slight reduction in the inhibitory ability was observed when residue M44 was substituted with alanine

The skin-specific mechanisms of GHK-Cu include stimulation of Type I and Type III procollagen synthesis, increased production of dermatan sulfate and other glycosaminoglycans, activation of the enzyme lysyl oxidase (which cross-links newly formed collagen), reduction of matrix metalloproteinase-1 (MMP-1, collagenase) activity, and anti-inflammatory suppression of TNF-alpha-induced IL-6 secretion

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Next, we employed a fluorescence polarization assay (FPA) to pinpoint the sequences crucial for binding among the specified regions, subsequently improving the cell applicability of the peptide sequence

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