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The network was formed between the pathway and gene, where the interactions were treated as directed, and the network was interpreted in the form of node and edge counts, with the edge betweenness by degree and neighborhood connectivity

Furthermore, a comparison of the surface properties of the bacterial and hNAPRT proteins revealed the presence in hNAPRT of an arginine-rich stretch ( 65 R FL R AF R L R ) forming a large mouth-like positively charged area on the top of the dimer, which is absent in the bacterial ortholog, but is present in a similar form in NAMPT, and could be involved in the binding to TLR4 (124)

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This leads to inadequacy of B 12 for cells

doi: 10.1016/j.chest.2017.04.170 190 TsaiIFKuoCPLinABChienMNHoHTWeiTYet al

Two million macrophages were seeded per well in a 6-well plate and maintained in culture for one week, with fresh media added every three days