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Genomic DNA contamination was eliminated using RNase-free DNase

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Nevertheless, although the cytochrome P450 pathway generates toxic species from trichloroethylene and tetrachloroethylene (Bull et al., 2002), a major contributor to the overall toxicity of trichloroethylene and tetrachloroethylene is the corresponding cysteine S -conjugate despite the fact that the glutathione S -conjugation pathway is quantitatively minor (Dekant, 2003)