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Additional hurdles include the risk of off-target effects in mtDNA or the nuclear genome, potential immune responses to the editing machinery, and substantial technical and regulatory barriers that complicate translation of in vivo gene editing into clinical practice

If no drug resistant alleles are present when a new drug is introduced, then there is a waiting time for them to arise and a low probability of fixation

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When there is uncertainty about the diagnosis, especially in cases where histological findings are unclear, a skin biopsy performed by an experienced pathologist can help establish the correct diagnosis

If there are bubbles, hold both the bottle and syringe in one hand, and tap the syringe with your other hand

To enhance interpretability despite this variability, we pooled outcomes within instruments (HDRS, BDI, MADRS), conducted prespecified subgroup analyses by comparator group and age, used random-effects models, performed leave-one-out sensitivity analyses and publication-bias diagnostics, and applied meta-regression to examine treatment duration and daily dose