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Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3) cultured in LB medium, after induction with 0.2 mM IPTG overnight at 16 C, the bacteria were pelleted by centrifugation at 4000 g for 10 min

V.LuoB.WangX.et al (2014)

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Basically, sailors, soldiers, prisoners, and even farm animals fed homogeneous, restricted, and/or limited diets became at risk for disease. However, proving exactly what caused these diseases proved to be elusive

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These metabolites were introduced to our in vitro infection model, to analyze their cytotoxic potential in isolation