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To initially locate the most abundant phosphorylation sites within ArfGAP1, we conducted in vitro radioactive kinase assays using [ 33 P]--ATP together with immunopurified recombinant full-length FLAG-tagged human LRRK2 (WT, G2019S or D1994A) and a series of GST-tagged ArfGAP1 protein fragments (residues 1-136, 137-415, 137-251, 252-359 and 360-415) ( Fig

The standard preparation step described in published peptide research literature is reconstitution with bacteriostatic water (BAC water)

Areas of investigation include: These early-phase studies are exploratory in nature and not predictive of applied neurological effects