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FRAP values are determined by measuring the increase in absorbance at 593 nm and comparing it to a ferrous ion standard solution or an antioxidant standard solution

After separation by SDS-PAGE, target proteins were transferred to PVDF membranes, blocked using protein-free rapid blocking solution, and then successively incubated with primary and secondary antibodies

More commonly, AC is used as the first capture step, the followed by one or more steps to remove remaining impurities

Introduction The immune system, an intricate network of cells including macrophages, neutrophils, dendritic cells, and natural killer (NK) cells ( Macrophages can be broadly classified into two categories: monocyte-derived macrophages, which originate from the bone marrow, circulate in the bloodstream, and subsequently extravasate into tissues, where they differentiate into macrophages

Methods: A literature search was performed using PubMed and Web of Science

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