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In summary, the kinetic data for the E170X mutants support a glutaredoxin-catalysed formation of GSSEtOH as well as an exclusive interaction between Glu170 and the glutathione moiety of GSSR in accordance with the glutathione-scaffold model

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In the end point mode, mixing the sample or standard with the supplied Detection Reagent and GSH yields a fluorescent product which is read at 460nm in a fluorescent plate reader with excitation at 390nm

Free Radical Biol Med 2002;33:1290300

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