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K7M2-Luciferase (K7M2-Luc) cells are cancer cells that express the reporter gene luciferase, which reacts with its substrate (luciferin) to generate chemiluminescence

In contrast, Gln deprivation abrogates c-Myc-mediated transcriptional repression, resulting in a significant upregulation of miR-29b-3p expression

But there is actually an optional fourth step that you can do after youve collected your protein off of the column: washing and regenerating the beads so that you can use them again

Previous studies revealed that Ca applications regulate the uptake of heavy metal ions as it competes for transporter sites on plasma membrane [24]

Chiu CC, Keeling CI, Bohlmann J

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