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Both supernatant and input samples were mixed with 4 L of 5 M NaCl and incubated at 65 C overnight to reverse the cross-linking, followed by digestion with 1 L Proteinase K (20 mg/ml) (Invitrogen, CA, USA) at 37 C for 1 h

A Powerful Antioxidant N-acetylcysteine, derived from the simple amino acid cysteine, provides significant protection against a broad array of modern toxins

15 YokotaSKubotaHMatsuokaYNaitohMHirataDMinotaSet alPrevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease

N Engl J Med 1994;330:10291035

I experienced mild disorientation lasting a few min three days after the vaccine

F., Cabrini, M