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Four to seven young adults (day 1 of adulthood) were picked into 50100 l S-basal (5.85 g NaCl, 6 g KH 2 PO 4 , 1 g K 2 HPO 4 in 1 liter milliQ) or M9 buffer (3.0 g KH 2 PO 4 , 6.0 g Na 2 HPO 4 , 0.5 g NaCl, 1.0 g NH 4 Cl in 1 liter milliQ) in a watch glass

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Indeed, mitochondria can produce both NO, by the activity of a Ca 2+ -sensitive mitochondrial NOS (mtNOS) (140, 528), and superoxide, following the partial reduction of oxygen within the mitochondrial matrix due to the natural leak of electron from the respiratory chain

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