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As expected, PS-CHO@ATRA-SS-ATRA exhibited highest down-regulation of Sox2 , Nanog and Oct4 genes in all formulations, further confirming that the ALDH-activated nano-prodrug could effectively eliminate the CSC and reduce the CSC-enriched microenvironment

Mitochondrial GSH is chiefly important for detoxifying ROS generated through oxidative phosphorylation (6-8)

10.21769/BioProtoc.4369 Bio-protoc 144 TongY.SongF

The bloodbrain barrier (BBB) is a highly selective, a border between the blood circulation and the central nervous system (CNS), necessary for maintaining homeostasis and protecting the brain from hazardous substances (Daneman and Prat, 2015)