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After 48 h, 10 M puromycin was added to each experimental group to eliminate non-transfected cells

Targeted DDSs based on organic, inorganic, and biological platforms (e.g., liposomes/lipid nanoparticles, polymeric nanoparticles, carrier-free drug self-assembly systems, hybrid inorganicorganic nanomaterials, and biomimetic carriers such as exosomes and protein nanocages) can enhance tumor accumulation and reduce off-target toxicity through active ligandreceptor targeting, microenvironment-adaptive delivery, and controlled release triggered by internal cues (pH, enzymes, reactive oxygen species, hypoxia) or external stimuli (light, heat, magnetic fields)

The research team is composed of a clinical assistance group of 169 general practitioners and nurses, and a technical group of 22 health professionals including doctors of different specialities, nurses and pharmacists

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3. Use twice daily for best results

Be sure to use ground organic coffee and boil with pure water (not tap water) for 15 minutes and let cool