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For the analysis researchers included 12,150,535 mother-infant pairs from 2016 to 2019 and of these pairs, just over 9%, 7%, 6%, and just under 6% of the mums reported smoking cigarettes before pregnancy, and in the first, second, and third trimester, respectively

Cells were then re-suspended into fresh media and 2 ml of the resulting solution was pipetted into each well of a 96-well plate

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