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The curriculum teaches a best practice approach for smoking cessation and is based on current clinical recommendations, including the five As of smoking cessation, a process that includes: Asking every patient about tobacco use, Advising all smokers to quit, Assessing smokers willingness to quit, Assisting smokers with treatment and referrals, Arranging follow-up contacts

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ELISA Following exposure to CSE, the cell culture conditioned media was collected and stored at 20C until analysis

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