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The cell suspensions were incubated with 5 L of FITC-conjugated Annexin V (V13242, Thermo) for 15 min at room temperature without exposure to light, followed by washing and resuspension in binding buffer

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Only 4 samples presented values that were less than 10% lower than what was declared (-6%, -3.4%, -2.5% and -8%)

Your brain downregulates the extra receptors it grew during chronic exposure, gradually restoring normal dopamine balance

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