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Gene set enrichment analysis GSEA was performed by generating the Gene MatriX file (.gmx) by using published STAT3 activation signatures 69

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Although these researchers also found no significant differences in bronchial microbiome between stability and exacerbation using 16S rRNA sequencing and shotgun metagenomic sequencing, functional metabolic capabilities showed significant changes in several pathways, indicating specific changes in the lung microbiome in the progression of COPD [82]

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