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Long- but not short-term adult-onset, isolated GH deficiency in male mice leads to deterioration of beta-cell function, which cannot be accounted for by changes in beta-cell mass

Acetyl CoA acetyl coenzyme A ADP adenosine diphosphate ATP adenosine triphosphate BNIP3 Bcl-2 interacting protein 3 CaOx calcium oxalate CypD cyclophilin D ETC electron transport chain FADH 2 flavin adenine dinucleotide GSSG glutathione disulfide H 2 O 2 hydrogen peroxide mGSH mitochondrial glutathione MIM mitochondrial inner membrane MOM mitochondrial outer membrane mtDNA mitochondrial DNA NADH nicotine adenine dinucleotide O 2 superoxide ROS reactive oxygen species TCA tricarboxylic acid Abbreviations References 1 AbateM.FestaA.FalcoM.LombardiA.LuceA.GrimaldiA.et al

Sperm suspensions were then homogenised by careful flicking of the Eppendorf tube, and a sub-sample was diluted 1:4 in milli-Q water (10L of thawed treatment sample in 40L of milli-Q water) to activate sperm by lowering osmolality

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After incubation, the blot was washed 3 times with 1 TBST for 15 min each at RT

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