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As RNA polymerase synthesizes mRNA, ribosomes can attach to the emerging transcript and begin translation before transcription is complete

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To investigate whether ROS was host or pathogen generated, a separate experiment was conducted in which cells were infected with 1 MOI zoospores (T#5), and either: 1) supplemented with 2.5 mM cysteine on day 3 for 8 hr, 2) incubated at 27C for 8 hr, 3) supplemented with 1.5 mM cysteine and incubated at 30C for 6 hr

Changes in absorbance per minute were converted to (moles of conjugated substrate min -1 mg enzyme -1 using the molar extinction coefficient for CDNB 340 = 9.6 mM 1 cm 1